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. 2021 Jun 8;22(2):851. doi: 10.3892/etm.2021.10283

Figure 4.

Figure 4

STK10 KO downregulates the phosphorylation level of p38 MAPK and ERM. (A) Western blot analysis showed decreased p38 MAPK and ERM phosphorylation, and increased levels of ZEB1, E-Cadherin, Cyclin D1 and Cyclin D3, but no change in activation of ERK or PI3K in the STK10-KO DU145 cells. (B) DU145 wild-type cells were transfected with the indicated quantity of STK10 expression vectors (0, 1, 2 and 3 µg) or empty vectors (the total quantity of vectors was 3 µg). The phosphorylation levels of p38 MAPK and ERM were assayed using western blotting. (C) DU145 control and STK10-KO cells were treated with EGF (50 ng/ml) for the indicated times (0, 5, 15 and 30 min). The phosphorylation levels of p38 MAPK and ERM were assayed using western blotting. (D) DU145 control and STK10-KO cells were transfected with 3 µg STK10 expression vectors or empty vectors. The phosphorylation levels of p38 MAPK and ERM were assayed using western blotting. STK10, serine threonine kinase 10; KO, knockout; ERM, ezrin-radixin-moesin; ZEB1, zinc finger E-box binding homeobox 1; EGF, epidermal growth factor; p-, phospho-; ZEB, zinc finger E-box-binding homeobox 1; p110, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit.