Figure 7.

Significant differential expression in tumor versus normal tissue of a new candidate isomiR set after batch correction. (A) Comparison of fold changes of expression in tumor versus normal before and after batch correction leads to a subset of isomiRs whose significant expression can only be observed after batch correction. (significance: log2 FC difference between tumor and normal amples > |0.4|, q-value < 0.05) (B) IsomiRs which are significantly differentially expressed either only before or only after batch correction show an expression spectrum well spread within all other isomiRs. For more information on significance see (A). (C) Roles in cancer of the canonical versions of the 19 isomiRs which are only significant after correction. If roles were controversial between tumor types, we focused on the role in lung cancer.As 5′ isomiRs have altered target spectra to the canonical versions, they were generally defined as ‘not classified’. 3′ isomiR names are colored in blue, 5′ in red and canonical isomiR names in green. TSM = tumor suppressor isomiR.