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. Author manuscript; available in PMC: 2021 Jun 17.
Published in final edited form as: Prog Retin Eye Res. 2018 Mar 22;65:28–49. doi: 10.1016/j.preteyeres.2018.03.003

Figure 3. Efficency of CRISPR based genome editing.

Figure 3.

A-B: Immunohistochemical analysis of GFP expression (green) following transduction of human retinal explants with AAV5-GFP at 1 week post-subretinal delivery. Unlike in vitro HEK293 transfection efficiency, which is near 100%, AAV5 based gene delivery vectors typically transduce less that 50% of the photoreceptor cells targeted. C: In vitro NHEJ efficiency. Of 40 sgRNAs targeting ten independent genes associated with inherited retinal degenerative disease, an average NHEJ efficiency of 20.6 ± 1.3% NHEJ was detected.