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. Author manuscript; available in PMC: 2021 Jun 17.
Published in final edited form as: J Bone Miner Res. 2019 Feb 28;34(5):955–963. doi: 10.1002/jbmr.3674

Figure 5.

Figure 5.

Effect of RGS5 on CASR signaling. HEK-CASR cells were transiently transfected with pcDNA3.1 control or RGS5 plasmid. Expression of RGS5 did not affect the total (A&B) or surface expression (C) of CASR as measured by WB and on-cell ELISA, respectively. D. Kinetic of CASR-mediated intracellular Ca2+ release was monitored 20 seconds before and 100 seconds after stimulation with 1.8 mM extracellular Ca2+. E. Concentration response curves of CASR-mediated intracellular calcium release were derived by GraphPad software as described in methods. Data in B-E are mean ± SEM from 5–6 independent experiments conducted in triplicate. T-test was used for data analysis. *P < 0.05, **P < 0·01, ***P < 0·001, ****P < 0·0001. (n.s. not significant).