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. 2021 Jun 4;12(15):4686–4697. doi: 10.7150/jca.58698

Figure 4.

Figure 4

PTX3 in gastric cancer cells reduces M2 macrophages polarization and increases M1 macrophages polarization in vitro. (A) The co-culture system of gastric cancer cells and macrophages which mimics the environment of milky spots metastasis. (B, C) BGC-823 and SGC-7901 cells were transfected with PTX3 overexpression plasmid (PTX3) or a negative control plasmid (CTL), then co-cultured with PMA-treated macrophages. The expressions of CD206 and CD86 were analyzed by flow cytometry. (D, E) The two gastric cancer cells and macrophages were treated as indicated in (B, C), the expressions of CD206 and CD86 in macrophages were detected by Immunofluorescence staining (Scale bars: 50μm) (Arrows point the cells of interest). (F) Gastric cancer cells and macrophages were treated as indicated in (B, C), the mRNA expressions of M1 markers (IL1β, TNF-α, CXCL9, CCR7) and M2 markers (Arg1, IL10, TGF-β and CCL22) was investigated by qRT-PCR (*P < 0.05, **P < 0.01, ***P < 0.001).