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. 2021 May 27;12(15):4463–4477. doi: 10.7150/jca.60008

Figure 3.

Figure 3

MST4 represses EMT in HCC cells. (A) Phase-contrast micrograph of the morphology of vector- or dnMST4-expressing Bel-7402 cells and Bel-7404 cells. (B) Western blot analysis for the detection of the indicated protein expression in dnMST4-expressing Bel-7402 cells and Bel-7404 cells. (C) Western blot analysis for the detection of the indicated protein expression in MST4-overexpressing Bel-7402 cells and Bel-7404 cells. (D) Immunofluorescence images showing the expression of EMT marker proteins of dnMST4-expressing Bel-7402 cells. Blue: DAPI. Scale bar, 20 µm. (E) Representative pictures of tumorsphere in dnMST4-expressing and MST4-overexpressing Huh7 cells. (F) The number of tumorspheres in dnMST4-expressing and MST4-overexpressing Huh7 cells. (G) Representative pictures of H&E and IHC staining for EMT markers in SHC specimens. (H) The number of cases with high or low indicated protein expression in SHC specimens. Data are mean ± SEM (*P< 0.05, **P <0.01, ***P <0.001).