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. 2021 Jun 1;12(15):4626–4637. doi: 10.7150/jca.59740

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Elevated expression of HOXA5 is associated with tamoxifen resistance in MCF7 breast cancer cells. (A) Reverse transcription-quantitative PCR (RT-qPCR) analysis of HOXA5 expression in MCF7 and tamoxifen-resistant breast cancer (TAMR) cells. GAPDH was used to normalize changes in HOXA5 expression levels between the two cell lines. (B) Western blotting of HOXA5 in MCF7 and TAMR cells. (C) Kaplan-Meier analysis of overall survival (OS) in estrogen receptor-positive (Luminal A and Luminal B) breast cancer patients with and without tamoxifen treatment. (D) RT-PCR analysis of HOXA5 in TAMR cells transiently transfected with pooled siHOXA5 for 48 hrs. (E) Cell viability curve of cells treated with siHOXA5 in the absence (left panel) and presence of 12 μM tamoxifen (right panel) at days 1, 2, and 3. β-Actin was used as an internal control for RT-PCR and western blotting. All experiments were performed in triplicate. ** p < 0.01, *** p < 0.001 compared with siCON by Student's t-test.