SARS-CoV-2 S treatment of macrophages at 39.5 °C can activate the NF-κB signaling pathway. (A-C) Western blotting analysis showed that p-NF-κB p65 and p-IκBα expression was increased following stimulation of SARS-CoV2 S-RBD in PBAMs (A), RAW264.7 cells (B), and THP-1 cells (C). (D-F) Western blotting analysis showed that p-NF-κB p65 and p-IκBα expression was decreased in the ShTRPV2 group compared with the control groups in PBAMs (D), RAW264.7 cells (E), and THP-1 cells (F), respectively. (G-I) Western blotting analysis showed that p-NF-κB p65 and p-IκBα expression was decreased in the SKF-96365 group compared with control groups in PBAMs (G), RAW264.7 cells (H), and THP-1 cells (I), respectively.