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Sirt5 interacts with DDX3 in an enzyme activity-dependent manner. (A) Immunoblot analysis of co-immunoprecipitation and cell lysate from HEK293T cells transfected with DDX3, Sirt5, and Sirt5-H158Y with indicated antibodies. (B, C) Immunoblot analysis of co-immunoprecipitation and cell lysate from Sirt5+/- and Sirt5-/- BMDMs with indicated antibodies. Data are representative of three independent experiments.
