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. 2021 May 24;11(15):7235–7246. doi: 10.7150/thno.52934

Figure 7.

Figure 7

Sirt5 demalonylates DDX3. (A) Luciferase activity in HEK293T cells transfected with IFN-β luciferase reporter, TBK1, and IRF3-5D, together with empty vector (Mock), Sirt5 or Sirt5-H158Y (kinase dead mutants). Results are expressed relative to Renilla luciferase activity. (B) Immunoblot analysis of immunoprecipitated DDX3 from cell lysate of Sirt5+/- and Sirt5-/- BMDMs (left), or Sirt5 stably overexpressed or empty vector RAW264.7 cells (right) infected with VSV for indicated time and detected with indicated antibodies. (C) Co-immunoprecipitation and immunoblot analysis of overexpressed DDX3 with or without Sirt5 using the indicated antibodies. (D) Luciferase activity in HEK293T cells transfected with IFN-β luciferase reporter, TBK1, and IRF3-5D, together with empty vector (Mock), and DDX3-WT and mutant. The results are expressed relative to the Renilla luciferase activity. Data are presented as mean ± SD of three independent experiments or representative of three independent experiments. (* P < 0.05).