Figure 1.
MRPL52 is upregulated in human BC. (A) Screening rationale of the RNA-seq assay. The screened genes were upregulated by more than 2-fold in breast cancer (BC) tissues and BC tissues with > 3 lymph nodes metastasis (TM) compared with adjacent normal tissues (ANTs) and BC tissues without lymph node metastasis (TnM), respectively. (B)The Bubble Chart of the results of GSEA was generated using the R package 'ggplot2'. Red represents a P value < 0.05 and redder colors indicate lower P values. (C) RT-qPCR of MRPL34, MRPL4, MRPL52, MRPL55, MRPL43 and MRPS24 expression in 30 human BC tissues and their paired ANTs. Data are shown as mean ± SD. (D) RT-qPCR of MRPL52 expression in 102 human BC tissues and their paired ANTs. Data are shown as mean ± SD. (E) MRPL52 expression in unpaired BC tissues and ANTs from clinical samples. Higher Ct value indicated lower gene expression. Data are shown as mean ± SD. (F) MRPL52 expression in TnM and TM tissues from clinical samples. Data are shown as mean ± SD.