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. 2021 May 25;11(15):7337–7359. doi: 10.7150/thno.57804

Figure 5.

Figure 5

MRPL52 mediates hypoxia-induced EMT, migration and invasion of BC cell. (A) Ability of cell migration and invasion tested by Transwell assay (mean ± SD, n = 3). *P < 0.05. Scale bars, 50 µm. (B) The morphological changes of cells undergoing EMT include the acquisition of spindle-liked phenotype and the loss of polarity and cell-cell adhesion, which are observed by an optical microscope. Scale bars, 50 µm. (C) The expression levels of Snail, Slug, ZEB-1 and ZEB-2 under hypoxia assessed by RT-qPCR (mean ± SD, n = 3). *P < 0.05. (D) The protein expression of MRPL52 and Snail was evaluated by WB. (E) WB and (F) IF were performed to assess the protein expression levels of Snail, E-cadherin, N-cadherin, ZO-1 and Vimentin. Scale bars, 50 µm.