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. 2021 Jun 1;11(15):7507–7526. doi: 10.7150/thno.59546

Figure 5.

Figure 5

CircRHOBTB3 retards CRC cell metastasis by mediating PTBP1. (A and B) Transwell and wound healing assays demonstrated that the effects of circRHOBTB3 overexpression on the migration and invasion of HCT116 and Colo320 cells were partly abrogated by PTBP1 overexpression (scale bar, 100 µm). (C and D) Transwell and wound healing assays demonstrated that the effects of circRHOBTB3 knockdown on the migration and invasion of DLD-1 and SW480 cells were partly abrogated by PTBP1 knockdown (scale bar, 100 µm). (E) Western blotting assays were applied to determine the rescue abilities of PTBP1 on circRHOBTB3 overexpression or knockdown in CRC cells. The protein levels of E-cadherin, N-cadherin, CD44, and PKM1/2 were determined. The data are presented as the mean ± SD of three independent experiments, two-tailed Student's t-tests, *p < 0.05, **p < 0.01 and ***p < 0.001.