Table 1. Summary of results from zebrafish studies evaluating the contribution of primitive myeloid cells to inflammatory cytokines involved in HSC emergence.

Intervention	Cells affected	Effect on HSPC	Effect on cytokines	Reference
Irf8a morpholino	↓ Csf1ra+ macrophages	↓ Runx1+ in AGM		Li et al., 2014
		↓ Cd41:Gfp+ in CHT
	↓ Mpeg1+ macrophages	↑ Runx1+ in AGM	↑ TNFα	Espín-Palazón et al., 2014
		↑ Kdrl+;cMyb+ in AGM
	↓ Mfap4+ macrophages	No effect	↓ IL-1β; ↓ G-CSF; ↑ TNFα	He et al., 2015
	↑ Lyz+ neutrophils
	↑ Mpx+ neutrophils	↑ Runx1+ in AGM	↑ IL-6	Tie et al., 2019
Cebp1b morpholino	↓ Lyz+ neutrophils	↓ Runx1+ in AGM	↓ IL-1β; ↓ G-CSF; ↓ TNFα	He et al., 2015
Spi1bc morpholino	↓ Mfap4+ macrophages	↓ Runx1+ in AGM	↓ IL-1β; ↓ G-CSF; ↓ TNFα	He et al., 2015
	↓ Lyz+ neutrophils
Spi1bd morpholino	↓ l-Plastin+ leukocytes	↓ Kdrl+;cMyb+ in AGM	↓ TNFα	Espín-Palazón et al., 2014
	↓ Mpx+ neutrophils
	↓ l-Plastin+ leukocytes	↓ Runx1+ in AGM	↓ IL-6	Tie et al., 2019
Spi1ae/Spi1bd morpholino	↓ Irf8+ macrophages	↓ Runx1+ in AGM	Unknown	Li et al., 2014
	↓ Mpo+ granulocytes	↓ Cd41:Gfp+ in CHT
	↓↓ Mfap4+ macrophages	↓ Runx1+ in AGM	Unknown	Frame et al., 2020
	↓ Mpx+ neutrophils	↓ Cd41:Gfp+ in CHT
Mpeg:NTRf + MTZ	↓ Mpeg1+ macrophages	↓ cMyb+ in CHT	Unknown	Frame et al., 2020
Mpeg:Dnikbaag	↓ Mpeg1+ macrophages	↓ cMyb+ in CHT	Unknown	Frame et al., 2020

^aIrf8: 5′-AAT​GTT​TCG​CTT​ACT​TTG​AAA​ATG​G-3′ and 5′-TCA​GTC​TGC​GAC​CGC​CCG​AGT​TCA​T-3′.

^bCebp1: 5′-GTC​AGA​CAC​CGA​CAT​GGC​TGT​GTG​T-3′, 5′-GGA​GCT​GCT​GAA​CTC​TAC​TCG​ATC​T-3′, and 5′-GTC​TGA​CTC​CGT​CAT​CGC​TGA​GTG​T-3′.

^cPu.1: 5′-CCT​CCA​TTC​TGT​ACG​GAT​GCA​GCA​T-3′ and 5′-GGT​CTT​TCT​CCT​TAC​CAT​GCT​CTC​C-3′.

^dPu.1/Spi1b: 5′-GAT​ATA​CTG​ATA​CTC​CAT​TGG​TGG​T-3′.

^eSpi1a: 5′-AGC​GAC​TCA​CGC​TGT​GGA​GGA​ACT-3′.

^fMpeg:NTR: Tg(mpeg1:GAL4;UAS:NTRmCherry) + metronidazole.

^gMpeg:Dnikbaa: Tg(mpeg1:GAL4;UAS:nfsB-mCherry;UAS:dnikbaa).