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. Author manuscript; available in PMC: 2021 Jun 17.
Published in final edited form as: J Parasitol. 2015 Jan 20;101(3):351–357. doi: 10.1645/14-606.1

Figure 5.

Figure 5.

Mean miracidial transformation rates after 24 hr in 1/3-diluted Helisoma trivolvis (dark-shaded columns) and Lymnaea elodes (light-shaded columns) mucus after heat-treatment (HT) (A) or proteinase K (PK) digestion (B). PK treatment controls include those for incubation temperature (Temp Control) and bead specificity (Bead Control) (see Materials and Methods for details). Untreated mucus (1/3) and buffer only (CBSS) served as positive and negative assay controls, respectively. HT had no significant effect on larval transformation rates in either snail species by 2-factor ANOVA with replication, while a significant PK treatment effect was detected for H. trivolvis mucus only. In this case, means with different letters are significantly different at the P < 0.05 level by Tukey’s means comparison tests. Here n = 3 for HT and n = 5 for PK experiments. Error bars denote standard errors.