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. 2021 Jan 25;48(3-4):kuab003. doi: 10.1093/jimb/kuab003

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© The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 3. — (A) Currently available heterologous hosts as E. coli, yeast, Streptomyces, and cyanobacteria strains for cyanobacterial NP production and major approaches for host engineering. PAM: photospacer adjacent motif; SgRNA: synthetic guise RNA. (B) The overproduction of shinorine in yeast included multicopy delta integration of biosynthetic genes, the overexpression of xylose assimilation genes (XL1, XL2, and XL3) from Scheffersomyces stipites, and the modulation of the pentose phosphate pathway through deleting TAL1 and overexpressing STB5 and TKL1 to overproduce the biosynthetic precursor sedoheptulose 7-phosphate (S7P).