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Chemical methods to detect O-GlcNAcylation. (a) GlcNAc and GalNAc analogues containing different functional groups that have been applied in metabolic labeling of O-GlcNAcylation. (b) The principle of Glyco-seek for sensitive quantification of O-GlcNAcylation. (c) O-GlcNAc imaging is enabled by O-GlcNAc metabolic labeling and EGFP fusion. EGFP: enhanced green fluorescent protein. TAMRA: carboxytetramethylrhodamine.
