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. Author manuscript; available in PMC: 2021 Jun 17.
Published in final edited form as: ACS Chem Biol. 2017 Jan 19;12(2):326–335. doi: 10.1021/acschembio.6b01065

Figure 4.

Figure 4.

Synthetic protein methods facilitating the functional investigation on O-GlcNAcylated proteins. (a) Native chemical ligation and expressed protein ligation methods for the synthesis of O-GlcNAcylated α-synuclein protein. (b) In a different strategy, GlcNAc-thiol or halogenated O-GlcNAc can be attached to dehydroalanine (Dha) through Michael addition or radical reaction, respectively. The S-GlcNAc on Cys112 of histone H2B promotes binding with the FACT protein complex, as well as histone ubiquitination by BRE1A enzyme. U: ubiquitination. FACT: facilitates chromatin transcript.