Table 3. Summary of studies assessing ABFT genetic structuring between main spawning areas (GOM and MED) through individuals of early life stages (larvae or young-of-the-year YOY) ensuring correct management unit assignment.
| Reference | Genetic tool | ABFT stock4 | Fixation index5 | |
|---|---|---|---|---|
| Western Atlantic | Eastern Atlantic | |||
| (Carlsson et al., 2007) | mtDNA sequence1 | Gulf of Mexico (GOM) Larvae (40) Year 2003 |
Mediterranean Sea (MED) YOY (107) Years 1998, 1999, 2000, 2001, 2002. |
0.013* |
| Microsatellite loci2 | GOM Larvae (40) Year 2003 |
MED YOY (280) Years 1998, 1999, 2000, 2001, 2002. |
0.006*** (8 loci) | |
| (Puncher et al., 2018) | SNP panel3 | GOM (64) and Cape Hatteras (16) Larvae (64) and YOY (16) Years 2007, 2008, 2009, 2010. |
MED Larvae (63) and YOY (350) Years 2008, 2011, 2012, 2013. |
0.008* (95 SNP panel) 0.014* (58 SNP panel) 0.034*** (24 SNP panel) |
| (Rodríguez-Ezpeleta et al., 2019) | SNP panel3 | GOM (26) and Slope Sea (13) Larvae (26) and YOY (13) Years 2007, 2008, 2009, 2010. |
MED Larvae (48) and YOY (117) 2008, 2011, 2012, 2013. |
0.004 (n.i.) |
| This study | mtDNA sequence1 | GOM Larvae (22) Year 2014 |
MED Larvae (21) Year 2014 |
0.029* |
| Microsatellite loci2 | GOM Larvae (62) Year 2014 |
MED Larvae (50) Year 2014 |
0.009* (8 loci) 0.010** (6 loci) 0.011**(4 loci) |
|
Notes:
The control region was sequenced in 847 bp (Carlsson et al., 2007) or 361 bp (this study).
Loci Tth5, Tth8, Tth10, Tth21, Tth34, Ttho-1, Ttho-4, and Ttho-7 were genotyped in Carlsson et al. (2007), and for this study we analyzed loci Tth208, Tth1-31, Ttho7, Tth34, Ttho4, Ttho1, Tth157, and Tth16-2.
SNPs derived from genomewide search for spatially informative loci by restriction site-associated DNA sequencing (RAD-seq).
Phase of early life stage (number of individuals) and collection year.
Significant probability obtained by 999 random permutations (*p < 0.05, **p < 0.01 and ***p < 000.1), n.i. not indicated. For nuclear markers FST is indicated. For mtDNA PhiST was calculated in Carlsson et al. (2007), we calculated analogous PhiPT for this study.
p < 0.05.
p < 0.01.
p < 000.1.
For each study the genetic tool and features of the collection of ABFT individuals used to characterize genetic diversity are summarized. The reported fixation indexes from pairwise comparison between ABFT collected in Western or Eastern stocks is compiled in the last column.