Table 1.
European and US Serodiagnostic Tests Evaluated in This Study
| Test | Source | Test Name | Manufacturer | Antibody Isotype Detected | Antigen Source |
|---|---|---|---|---|---|
| ELISAs | European | Enzygnost Borreliosis | Siemens/Dade Behring, Marburg, Germany | IgM | Lysate of Borrelia afzelii strain PKo |
| Enzygnost Lyme Link VlsE/IgG | Siemens/Dade Behring, Marburg, Germany | IgG | Lysate of B. afzelii strain PKo, plus rVlsE derived from B. afzelii, Borrelia garinii, and Borrelia burgdorferi s.s. | ||
| US | Wampole B. burgdorferi IgG/IgM ELISA II | Alere Inc, Waltham, MA | IgM, IgG | Lysate of B. burgdorferi s.s. strain B31 | |
| C6 B. burgdorferi | Immunetics, Inc, Boston, MA | IgM, IgG | 25-mer amino acid peptide corresponding to IR6 of B. burgdorferi s.s. strain B31 | ||
| Immunoblots | European | Borrelia MiQ + VlsE IgM test kit | Viramed Biotech AG, Planegg, Germany | IgM | Extracts of B. afzelii strain PKo and B. burgdorferi strain B31 combined, supplemented by direct application of rVlsE derived from B. burgdorferi s.s. strain B31 |
| Borrelia MiQ + VlsE IgG test kit | Viramed Biotech AG, Planegg, Germany | IgG | Extracts of B. afzelii strain PKo and B. burgdorferi s.s. strain B31 combined, supplemented by direct application of rVlsE derived from B. burgdorferi s.s. strain B31 | ||
| US | Borrelia B31 ViraBlot IgM test kit | Viramed Biotech AG, Planegg, Germany | IgM | Extract of B. burgdorferi s.s. strain B31 | |
| Borrelia B31 plus VlsE ViraBlot IgG test kit | Viramed Biotech AG, Planegg, Germany | IgG | Extract of B. burgdorferi s.s. strain B31, supplemented by direct application of rVlsE derived from B. burgdorferi s.s. strain B31 |
Abbreviations: ELISA, enzyme-linked immunosorbent assay; IgG, immunoglobulin G; IgM, immunoglobulin M; rVlsE, recombinant VlsE; s.s., sensu stricto; VlsE, Vmp-like sequence, expressed.