Overview of imaging mass spectrometry ion sources discussed. (A) Matrix-assisted laser desorption/ionization, (B) liquid extraction surface analysis (LESA), (C) laser ablation electrospray ionization (LAESI), (D) desorption electrospray ionization (DESI), (E) liquid microjunction (LMJ), and (F) infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI).