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. 2021 Jun 7;17(6):e1009662. doi: 10.1371/journal.ppat.1009662

Fig 1. SIRPA has broad antiviral activity.

Fig 1

A) U2OS, 293T and NR-9456 cells were transfected with the indicated siRNAs and infected with the indicated viruses 48 hours later. RNA was isolated at 14 (VSV, mNoV) or 24 (JUNV-C1, TCRV, LCMV, ZIKV) hpi, and DNA was isolated at 24 (HSV-1) or 48 (MLV) hpi. Viral nucleic acids were measured by qPCR. Shown is the average ± standard deviation (SD) of 3–6 independent experiments. Student’s T test was used to determine significance. **, P ≤ 0.005; ***, P ≤ 0.001; ns, not significant. B) U2OS and A549 cells were transfected with the indicated siRNAs and infected with pseudoviruses bearing the indicated viral glycoproteins. At 48 hpi, luciferase assays were performed. Bald viruses lacking any viral glycoprotein served as controls. Shown is the average ± SD of 3 independent experiments. One-way ANOVA was used to determine significance. **, P ≤ 0.001; ***, P ≤ 0.0003; ****, P ≤ 0.0001.