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. 2021 Jun 17;16(6):e0253393. doi: 10.1371/journal.pone.0253393

Table 2. Physiochemical, immunogenic and solubility properties of vaccine protein.

Parameter Value Comment
Molecular Weight (kDa) 50.914 Suitable
Theoretical PI 10.21 Basic in nature
Estimated Half Life 30 h Mammalian reticulocyte (in vitro)
>20 h Yeast (in vivo)
>10 h E. coli (in vivo)
Instability Index 29.55 (stable) Stable
Aliphatic Index 84.36 Thermostable
GRAVY 0.170 Slightly hydrophobic
VaxiJen Score 0.7042 Antigenic
AlgPred - Non-allergic
Protein-Sol 0.551 Highly soluble in E. coli