TABLE 1.

Genomic findings (TMB, microsatellite status, and mutations) for each biopsy

Gene identification	Patient 1	Patient 2	Patient 3	Patient 4	Patient 5	Patient 6, Biopsy 1	Patient 6, Biopsy 2	Patient 7	Aberration Details	Targetable Aberration
TMB	High, 56 Muts/Mb	High, 115 Muts/Mb	High, 31 Muts/Mb	Intermediate, 11 Muts/Mb	High, 25 Muts/Mb	Intermediate, 7 Muts/Mb	Intermediate, 12 Muts/Mb	High, 75 Muts/Mb	--	--
Microsatellite Status	Stable	Stable	Stable	Stable	Stable	Stable	Stable	Not noted	--	--
TP53	x	x	x	x	x	x	x	x	Table 2
BRCA2		x†		x†		x†	x†		Table 2	x (Patients 2, 4, and 6)
RB1		x			x	x	x		Table 2
FAT1	x		x					x	Table 2
CDKN2A	x			x				x†	Table 2	x (Patient 7)
KMT2C (MLL3)	x			x	x				Table 2
MLL2	x	x		x					Table 2
FLT1	x		x						Table 2
NOTCH1			x	x					Table 2
LRP1B		x						x	Table 2
NOTCH3	x		x						Table 2
BCORL1	x	x							Table 2
TERT-promoter				x				x	Table 2
ASXL1	x								W930*
JUN	x								Amplification
HRAS		x†							G13D	x
SMARCB1		x†							Splice site 629-1G>A	x
CIC		x							R422*
IKZF1		x							G158S, Q366*
PREX2		x							E421K
SPOP		x							E47K
EZH2			x†						Y646N	x
ARID2			x						Q651*
NOTCH2				x					Q206*
TNFRSF14				x					G124fs*66
FANCA					x				E692*
VHL					x				L188V
CCNE1						x			Equivocal
NFKB1A						x			Equivocal
NKX2-1						x			Equivocal
AKT1							x†		Amplification	x
PIK3R2							x		E296*
ERBB3								x†	R135C	x
NF2								x†	Q400*	x
CARD11								x	R888C
RUNX1T1								x	R394W

Redundant mutations (seen in more than one biopsy) are shown in green rows

^†Mutations identified as candidates for targeted therapy