Video 7. Representative examples of confocal time-lapse recording of EGL explant cultures of P4-P5 Plxnb2^fl/fl and En1^Cre;Plxnb2^fl/fl cerebella with 15 min interval, starting from DIV1.

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Cerebella were electroporated ex vivo with GFP to visualize individual CGNs and follow their migration over time (some striking examples are pseudo-colored). Control CGNs with a bipolar morphology migrate away from the explant in a straight direction. Plxnb2 mutant CGNs change their direction of migration multiple times and cover long distances in reverse direction (back to the explant).