Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain; strain background (Mus musculus) | En1Cre (C57BL/6J) | DOI:10.1101/gad.14.11.1377 |
En1tm2(cre)Wrst/J RRID:IMSR_JAX:007916 |
|
| Strain; strain background (Mus musculus) | Wnt1-Cre (C57BL/6J) | DOI:10.1002/dvdy.20611 | B6.Cg-E2f1tg(Wnt1-cre)2Sor/J RRID:IMSR_JAX:022501 |
|
| Strain; strain background (Mus musculus) | Plxnb2-/- | DOI:10.1523/JNEUROSCI.4710-06.2007 |
Plxnb2tm1Matl
RRID:MGI:4881705 |
Gift from Roland Friedel |
| Strain; strain background (Mus musculus) | Plxnb2 cKO | DOI:10.1523/JNEUROSCI.5381-06.2007
|
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| Antibody | Anti-Calbindin D-28k (Rabbit antiserum) | Swant | Cat# CB38, RRID:AB_2721225 |
IF(1:1000), |
| Antibody | Anti-Calbindin D-28k (Mouse monoclonal) | Swant | Cat# 300, RRID:AB_10000347 |
IF(1:1000) |
| Antibody | Anti-Foxp2 (N16) (Goat polyclonal) | Santa Cruz | Cat# sc-21069, RRID:AB_2107124 |
IF(1:1000) |
| Antibody | Anti-Glial fibrillary acidic protein (GFAP) (Mouse monoclonal) | Millipore | Cat# MAB360, RRID:AB_11212597 | IF(1:500) |
| Antibody | Anti-Green Fluorescent Protein (GFP) (Rabbit polyclonal) | ThermoFisher Scientific | Cat# A-11122, RRID:AB_221569 | IF(1:2000) |
| Antibody | Anti-Green Fluorescent Protein (GFP) (Chicken polyclonal) | Aves | Cat# GFP-1010, RRID:AB_2307313 | IF(1:2000) |
| Antibody | Anti-Phospho-Histone H3(Ser10) (Rabbit polyclonal) | Cell Signaling | Cat# 9701; RRID:AB_331535 | IF(1:1000) |
| Antibody | Anti-Ki67 (Rabbit polyclonal) | Abcam | Cat# Ab15580; RRID:AB_443209 | IF(1:500) |
| Antibody | Anti-Olig-2 (Rabbit monoclonal) | Millipore | Cat# AB9610; RRID:AB_10141047 |
IF(1:500) |
| Antibody | Anti-Pax6 (Rabbit polyclonal) | Millipore | Cat# AB2237; RRID:AB_1587367 |
IF(1:1000) |
| Antibody | Anti-Plexin-B2 (Armenian Hamster monoclonal) | Novus | Cat# NBP1- 43310; RRID:AB_10006672 |
IF(1:1000) |
| Antibody | Anti-Sema6A (Goat polyclonal) | R and D systems | Cat# AF1615; RRID:AB_2185995 |
IF(1:500) |
| Antibody | Anti-Contactin-2/TAG1 (Goat polyclonal) | R and D systems | Cat# AF4439; RRID:AB_2044647 |
IF (1:500) |
| Antibody | Anti- beta-Tubulin III (Rabbit polyclonal) | Sigma-Aldrich | Cat# T2200; RRID:AB_262133 |
IF (1:1000) |
| Antibody | Anti- VGLUT1 (Guinea pig polyclonal) | Millipore | Cat# AB5905; RRID:AB_2301751 |
IF (1:3000) |
| Antibody | Donkey Anti-Rabbit IgG (H + L) Alexa Fluor 488 | Jackson Immunoresearch | Cat# 711-545-152; RRID:AB_2313584 |
IF (1:750) |
| Antibody | Donkey Anti-Rabbit IgG (H + L) Cy3 | Jackson Immunoresearch | Cat# 711-165-152; RRID:AB_2307443 | IF (1:750) |
| Antibody | Donkey Anti-Rabbit IgG (H + L) Alexa Fluor 647 | Jackson Immunoresearch | Cat# 711-605-152; RRID:AB_2492288 | IF (1:750) |
| Antibody | Bovine Anti-Goat IgG (H + L) Alexa Fluor 647 | Jackson Immunoresearch | Cat# 805-605-180; RRID:AB_2340885 | IF (1:750) |
| Antibody | Donkey Anti-Goat IgG (H + L) Cy3 | Jackson Immunoresearch | Cat# 705-165-147; RRID:AB_2307351 | IF (1:750) |
| Antibody | Donkey Anti-Mouse IgG (H + L) Alexa Fluor 647 | Jackson Immunoresearch | Cat# 715-605-150; RRID:AB_2340862 | IF (1:750) |
| Antibody | Donkey Anti-Chicken IgG (H + L) Alexa Fluor 488 | Jackson Immunoresearch | Cat# 703-545-155; RRID:AB_2340375 | IF (1:750) |
| Antibody | Donkey Anti-Chicken IgY (H + L) Cy3 | Jackson Immunoresearch | Cat# 703-165-155; RRID:AB_2340363 | IF (1:750) |
| Antibody | Goat Anti-Armenian Hamster IgG (H + L) Alexa Fluor 488 | Jackson Immunoresearch | Cat# 127-545-160; RRID:AB_2338997 | IF (1:750) |
| Antibody | Goat Anti-Armenian Hamster IgG (H + L) Cy3 | Jackson Immunoresearch | Cat# 127-165-160; RRID:AB_2338989 | IF (1:750) |
| Antibody | Donkey Anti-Guinea Pig IgG (H + L) FITC | Jackson Immunoresearch | Cat# 706-095-148; RRID:AB_2340453 | IF (1:750) |
| Antibody | Donkey Anti-Guinea Pig IgG (H + L) Cy3 | Jackson Immunoresearch | Cat# 706-165-148; RRID:AB_2340460 | IF (1:750) |
| Antibody | Donkey Anti-Goat IgG (H + L) Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11055; RRID:AB_25341020 | IF (1:750) |
| Antibody | Donkey Anti-Goat IgG (H + L) Alexa Fluor 555 | Thermo Fisher Scientific | Cat# A21432; RRID:AB_2535853 | IF (1:750) |
| Antibody | Donkey Anti-Mouse IgG (H + L) Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A21202; RRID:AB_141607 | IF (1:750) |
| Other | Hoechst 33342 | Thermo Fisher Scientific | Cat# H3570 | IF (1:1000) |
| Commercial assay or kit | Click-iT EdU Cell Proliferation Kit for Imaging,Alexa Fluor 647 dye | Thermo Fisher Scientific | Cat# C10340 | |
| Chemical compound, drug | Gelatin | VWR Chemicals | Cat# 24350.262 CAS Number: 9000-70-8 | |
| Chemical compound, drug | Thimerosal | Sigma-Aldrich | Cat# T8784-5g CAS Number: 54-64-8 | |
| Chemical compound, drug | TritonX-100 | Sigma-Aldrich | Cat# X100-500ml CAS Number: 9002-93-1 | |
| Chemical compound, drug | SYBR Gold nucleic acid stain | Thermo Fisher Scientific | Thermo Fisher Scientific:S11494 | |
| Software, algorithm | Fiji | NIH | RRID:SCR_002285 | Analysis |
| Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 | Analysis |
| Software, algorithm | Imaris | Oxford Instruments | RRID:SCR_007370 | Analysis |
| Software, algorithm | iMovie | Apple | http://www.apple.com/fr/imovie/ | Analysis |