Figure 6.

The absence of CRY disrupts synchronized rhythms of individual oscillators under LD and LL measured in different cell groups of cultured fly brains. Bioluminescence recordings showing individual oscillations in cultured adult Drosophila brains under 8 d of control LD (n = 3 brains) or 8 d of LL (n = 3 brains), both followed by DD. A, XLG-PER-Luc individual oscillations (one colored trace indicates one cell) are highly synchronized in 8 d of LD, then gradually dampen in DD (s-LNv = 18 cells, l-LNv = 19 cells, LNd = 18 cells, DN1 = 27 cells, DN3 = 18 cells). B, cry^b XLG-PER-Luc individual oscillations (one colored trace indicates one cell) are high amplitude but desynchronized in 8 d of LD and in following DD (s-LNv = 17 cells, l-LNv = 20 cells, LNd = 11 cells, DN1 = 13 cells, DN3 = 25 cells). C, cry^b XLG-PER-Luc individual oscillations (one colored trace indicates one cell) are detectable but desynchronized in 8 d of LD and in following DD, with a single DN1 cell showing measurable oscillation (s-LNv = 29 cells, l-LNv = 20 cells, LNd = 16 cells, DN1 = 30 cells, DN3 = 29 cells).