Figure 7.
Heightened NMDA-mediated ROS production following ERβ blockade in isolated PVN ERβ-EGFP cells from AngII-infused females. A, In females, ROS production is increased in isolated ERβ-EGFP PVN cells treated with PHTPP compared with their Veh-treated counterparts. B, Example of isolated PVN cells from a female mouse showing fluorescence for EGFP, DHE, and merged EGFP/DHE. C, Compared with Veh, application of PHTPP results in a heightened NMDA-induced ROS production in isolated ERβ-EGFP cells. D, PHTPP-enhanced ROS in dissociated ERβ-EGFP PVN cells was blocked by the NOX2 inhibitor gp91ds but not by the gp91 scrambled peptide following 100 μm NMDA. C, D, The ratio of DHE was calculated as Ft/Fo, where Ft is DHE fluorescence in a given cell after application of NMDA, and Fo is the baseline DHE fluorescence of the same cell immediately before NMDA. *p < 0.05; **p < 0.01; factorial and repeated-measures ANOVA followed by Tukey post hoc. Scale bar, 20 µm. Data are mean ± SEM.