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. 2021 Jun 16;41(24):5287–5302. doi: 10.1523/JNEUROSCI.2566-20.2021

Figure 7.

Figure 7.

Inhibition of MCx excitatory neurons reverses the mechanical allodynia induced by MCx astrocyte activation in normal rats. A, Timeline of the experiments. B, The GFAP-M3-eGFP+CaMKII-M4-mCherry virus was bilaterally injected into the rat MCx. Green represents the GFAP-M3 virus expression of the eGFP tag. Red represents the CaMKII-M4 virus expression of the mCherry tag. Red-green merged image represents the expression of GFAP-M3-eGFP+CaMKII-M4-mCherry in the MCx. Scale bars, 100 µm. C, Mechanical pain hypersensitivity of rats expressing GFAP-M3-eGFP+CaMKII-M4-mCherry or GFAP-M3-eGFP+CaMKII-mCherry before CNO injection (0 h) and over a 6 h time course after CNO treatment. ***p < 0.001 versus the control group, n = 11 or 12 rats/group, two-way repeated-measures ANOVA followed by Dunnett’s multiple comparisons test. D, Maximal possible effects of selective activation of astrocytes in the MCx at 2 h after CNO injection on mechanical allodynia in naive rats. ***p < 0.001 versus the control group, n = 11 or 12 rats/group, two-way repeated-measures ANOVA followed by Dunnett’s multiple comparisons test.