Figure 5.

Integrated CT nerve responses to different concentrations of capric acid (30 s) (A,C) and various tastants (15 s) in WT mice and Gpr84^−/− mice (B and D, respectively). NH₄Cl (100 mm) was applied (15 s) before and after the session as a reference. Bars represent the duration of the stimulus. AUC of the integrated response for each tastant in WT and Gpr84^−/− mice was calculated and compared via unpaired two-tailed Student’s t tests. Responses were normalized to the response to 100 mm NH₄Cl. Responses show the mean ± SEM (n = 5-10 mice). No significant differences were observed between the WT (black bars) and KO (open bars) mice (F) when stimulated with NaCl (30 mm), MSG (10 mm), DB (1 mm), HCl (1 mm), and Steviose (25 mm); however, the nerve response to capric acid was significantly decreased at concentrations of 1 mm (t₍₈₎ = 2.64, p = 0.030, unpaired t test) and 5 mm (t₍₁₃₎ = 3.79, p = 0.002, unpaired t test) in GPR84^−/− mice (E). *p < 0.05, Significant differences between two groups.