Table 1.
Passage history and FCS deletion levels of England 02 stocks.
| Cell line used (source and reference) | Passage number | |||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| Vero E6 (ECACC, 85020206) | <1%d | |||||
| Vero E6 (BEI, NR-596)a | 93% | 100% | ||||
| Vero E6 (BEI, NR-596)a | 73% | |||||
| Vero\hSLAM (ECACC, 04091501)a | 1.2% | |||||
| Calu-3 (ATCC, HTB55)a | <1%d | |||||
| Calu-1 (ECACC, 93120818)a | <1%d | |||||
| Vero E6 (BEI, NR-596)b | 28% | 54% | 80% | |||
| Vero E6 (ECACC, 85020206)b | 40% | 64% | 80% | |||
| Vero\hSLAM (ECACC, 04091501)b | 8.7% | 4.3% | 13.1% | |||
| Calu-1 (ECACC, 93120818)a | 4.5% | 7.5% | ||||
| Vero E6 (ECACC, 85020206)c | 28% | 80% | 96% | |||
aDerived from the P1 stock propagated in Vero E6 (ECACC, 85020206).
bAll derived from the P2 stock propagated in Calu-1 cells.
cDerived from the P3 propagated in Calu-1 cells.
dNo deletions detected in over 99% of the reads spanning the FCS region.