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. 2021 Jun 17;11:12800. doi: 10.1038/s41598-021-92329-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Illustration of the RPA-Cas12a method for HR-HPVs detection. The system includes an RPA amplification with a pool derived from PGMY/GP6+ primer set that is able to amplify 13 types of HR-HPV in a reaction, and a Cas12a detection with a crRNA pool. The results can be achieved within 35 min using a fluorescent reader.