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. 2021 Jun 17;11:12800. doi: 10.1038/s41598-021-92329-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Serial dilutions of the indicated HPV plasmid for LoD determination. After RPA amplification at 37 °C for 20 min using 10,000 copies of plasmid for each reaction, 10 μL of the yield was transferred to 40 μL of the Cas12a mixture for cleavage assays. NTC no-template control.