Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 17;12:3687. doi: 10.1038/s41467-021-24067-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Time-lapse confocal microscopy images of the minus-end stability of daughter microtubules after branch-forming nucleation. γTuRC (magenta circles in the diagrams) and microtubules (green lines) are labeled by MZT1-GFP and mCherry-TUB6, respectively. Open and closed arrowheads respectively indicate the absence and the presence of MZT1 particles, whereas yellow and white arrows show the plus and minus ends of daughter microtubules. Kymographs track the dotted blue lines. Appearance of MZT1 particles set the zero time point. a In the ktn1 cells, the minus ends of daughter microtubules are stably attached to the branching nucleation sites, as seen in the kymograph at 1100 s after nucleation. A total of 298 similar events were observed. b In the wdr8 ktn1 cells, daughter microtubules are readily released even in the absence of katanin. A total of 63 similar events were observed. Bars, 2 µm.