Fig. 5. Wdr8 is not required for katanin localization during cell division.

a Colocalization of katanin and Msd1 during cell division. Time-lapse confocal microscopy images of GFP-KTN1 (green) and Msd1a-mCherry (magenta) in the dividing epidermal cells of the primary roots of 3-day-old wild-type seedlings. Beginning of the nuclear envelop breakdown in the root cell marked by the dotted yellow line sets the time zero points (minute : second). Katanin is substantially colocalized with Msd1 but accumulates more toward the minus ends of microtubules in spindles (12 : 00) and phragmoplasts (17 : 00). Four cytokinesis events from three plants were observed with similar results. Bar, 10 µm. b Subcellular localization of katanin during cell division. Time-lapse confocal microscopy images of GFP-KTN1 in the dividing epidermal cells of the primary roots of 3-day-old wild-type and wdr8 seedlings. Beginning of the nuclear envelop breakdown sets the time zero points (minute : second). Each images were averaged images of three z-slices taken with 0.7 µm step. A total of ten and eight similar events were observed in wild-type cells (from eight plants) and wdr8 cells (from eight plants), respectively. Bars, 5 µm.