Fig. 7. Mutations in the Msd1–Wdr8 complex complement the cell shape defects of katanin mutants.

a Eight-day-old Arabidopsis seedlings grown on a vertically placed agar medium. Bar, 10 mm. b Growth and statute of flowering Arabidopsis plants. Bar, 10 cm. c Growth rate (cm per day) measured by daily increment in the length of primary roots from 5 to 10 days after germination in wild type (20 seedlings), msd1a msd1b (20 seedlings), wdr8 (20 seedlings), ktn1 (19 seedlings), msd1a msd1b ktn1 (18 seedlings), and wdr8 ktn1 (18 seedlings). The bottom and top of each box represent the first and third quartiles, respectively; the horizontal line inside each box is the median (second quartile) and the red horizontal line represents the average. The whiskers range between 2nd percentile and 98th percentile. n.s., not significant and ***p < 0.0001, determined by the Steel–Dwass test. Exact p-values are indicated in parentheses. d Root tip morphology of indicated genotypes. Cortex cell files are highlighted with the turquoise paint. Bar, 100 µm. e Cortex cell shape of 4-day-old primary roots at the differentiation zone where cell expansion has ceased in wild type (98 cells from 4 seedlings), wdr8 (47 cells from 3 seedlings), ktn1 (94 cells from 2 seedlings), and wdr8 ktn1 (76 cells from 2 seedlings). Left, cell length and cell width of individual cells. Middle, cell length. Right, cell width. n.s., not significant and ***p < 0.0001, determined by the Steel–Dwass test. Exact p-values are indicated in parentheses. The elements of the box plot are defined as in c.