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. 2021 Jun 17;11:12769. doi: 10.1038/s41598-021-92105-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Summary diagram showing a conceptual framework of the trends observed in the biologic and geologic components of the Puquio system across an increasing gradient in electrical conductivity. Brine BAC refers to bacterial communities in the brines. E1 BAC refers to bacterial communities in the E1 layer. Thickness of diversity and abundance bars indicate general trends as supported in Figs. 3, 4 and Supplementary Figs. S2–S4. In cases where total abundance showed no apparent relation with EC (e.g. Phytoplankton, Zoobenthos, Brines BAC, and E1 BAC), the bars were subdivided to highlight the effect of EC on individual functional groups. AP anoxygenic phototrophs, SO sulfur oxidizers, S sulfate, N nitrate. Figure was created in Microsoft PowerPoint for Mac v16.48. https://www.microsoft.com/en-us/microsoft-365/powerpoint.