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. 2021 Jun 17;12:3684. doi: 10.1038/s41467-021-24010-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a CellTrace Violet (CTV)-labeled mouse splenic T cells were isolated and co-cultured with Hepa1–6 cells in the presence or absence of anti-Nectin2 neutralizing antibody (15 µg/mL). Mean ± SD is presented. b CTV-labeled T cells were cocultured with Hepa1–6 (WT), -Nectin2-KO1, -Nectin2-KO2, -Nectin2-KO3 cells. Mean ± SD is presented. c Representative picture and weight of Nectin2 WT (Nectin2WT:Tp53KO:c-MycOE), and Nectin2 KO (Nectin2KO:Tp53KO:c-MycOE) HCC tumors. Scale bar = 1 cm. d–f Numbers of tumor-infiltrating lymphocytes were analyzed by flow cytometry. g, h Representative pictures, and quantification of CD4 + T cells and CD8 + T cells in HCC tumors by IHC staining. Scale bar = 100μm in IHC representative pictures. a–h Student’s t test. The experiment was performed with a variable number of biologically independent samples (n number) (a n = 6, n = 3, and n = 3 for T cells only Ctrl and anti-Nectin2 respectively in CD4+ cells, and n = 3 for all groups in CD8+ cells; b n = 10, n = 6, n = 4, and n = 4 for WT, Nectin2-KO1, Nectin2-KO2, and Nectin2-KO3, respectively in both CD4+ and CD8+ cells; c–e, g: n = 7 per group; f, h: n = 21 per group). Source data are provided as a Source Data file.