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. 2021 Jun 17;11:12796. doi: 10.1038/s41598-021-92384-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Presence of mucosal enteric glial cells in human gut xenograft, despite the absence of luminal microbiota. Cryosections of human fully developed gut xenografts were stained with DAPI (A), anti Tuj1 (B) and anti S100β (C) antibodies. Individual channels are displayed in A–C and merged in D. Confocal images of gut cryosections were acquired with Leica TCS SP5 with a DN6000 microscope assisted by the LAS AF software. All images were processed with ImageJ (Wayne Rasband, NIH) using 3-D reconstructions and opacity mode. Scale bars 50 µm.