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. 2021 Jun 17;11:12796. doi: 10.1038/s41598-021-92384-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mucosal enteric glial cells are present in human gut that had been stripped of the myenteric ganglia at time of transplantation. When a portion of the fetal gut was transplanted after the myenteric plexus layer had been stripped off (black arrows in A,B), glial cells are present in the corresponding part of the developed xenograft (C,D). Formalin-fixed, paraffin embedded tissues were stained with anti-human S100β (B,D) and counterstained with haematoxylin. Scale bars; 500 µm (A), 50 µm (B,C) and 200 µm (D).