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. 2021 Jun 17;4:754. doi: 10.1038/s42003-021-02277-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Immunolabelling of cell cycle synchronized Mesotaenium caldariorum cells during semicell morphogenesis. a, b LM15 binding was found in the isthmus when cells were starting to divide. c, d LM15 is evident in the ends of daughter cells near the division zone (while chlorophyll is also evident and colored green in the middle of the two daughter cells as indicated by arrows) e, f cell expansion is almost completed of the new Mesotaenium caldariorum cells and LM15 labeling is very clear in the newly formed “primary cell wall”. g, h Cell division and expansion is completed and the “primary cell wall” is cast off as a single piece depicted here, including the LM15 labeling. Top panel: LM15 (TRITC) (green), lower panel: DIC light image. c Chlorophyll autofluorescence (green), in the middle of the cells. Scalebars as indicated in the DIC images: 20 µm.