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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2021 Apr 6;1863(7):183618. doi: 10.1016/j.bbamem.2021.183618

Fig. 3.

Fig. 3.

BMH cross-linking analysis of rHDL with apoE3 and apoE4 Cys variants. Discoidal rHDL bearing 10–15 μg apoE3 (left) or apoE4 (right) single Cys variants were treated with BMH in DMSO and electrophoresed on a 4–20% acrylamide gradient SDS-PAGE gel as described under Methods. The lane assignments for apoE3 variants (left) are as follows: Lane 1, molecular mass standards in kDa; lane 2, lipid-free protein; lane 3, rHDL; lane 4, rHDL + DMSO; lanes 5, 6, 7, and 8, rHDL+10-, 25-, 50- and 100- fold molar excess of BMH over apoE3, respectively. The lane assignments for apoE4 variants (right) are: Lane 1, molecular mass standards in kDa; lane 2, rHDL + DMSO; lane 3, 4, 5 and 6, rHDL+10-, 25-, 50- and 100-fold molar excess of BMH over apoE4, respectively.