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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2021 Apr 6;1863(7):183618. doi: 10.1016/j.bbamem.2021.183618

Fig. 4.

Fig. 4.

BM(PEG)3 cross-linking analysis of rHDL with apoE3 and apoE4 Cys variants. Discoidal rHDL bearing 10–15 μg apoE3 (left) or apoE4 (right) were treated with increasing concentration of BM(PEG)3 (in the direction shown) in DMSO and electrophoresed on a 4–20% acrylamide gradient SDS-PAGE gel as described under Methods. Two single Cys variants were selected to represent the NT (top) and CT (bottom) domains for each isoform. The lane assignments are as follows: Lane 1, molecular mass standards in kDa; lane 2, rHDL + DMSO; lanes 3 and 4, rHDL+10×–25× and 50×–100× molar excess of BM(PEG)3 over apoE, respectively.