Fig. 5.
Cross-linking analysis of rHDL with apoE(201–299) single Cys variants. Discoidal rHDL bearing 10–15 μg apoE(201–299) single Cys variants were treated with BMH in DMSO and electrophoresed on a 4–20% acrylamide gradient gel as described under Methods. The lane assignments are as follows: Lane 1, molecular mass standards in kDa; lane 2, DMSO; rHDL + DMSO; lanes 4, 5, 6, 7, 8 and 9, rHDL +2×, 5×, 10×, 25×, 50×, 100× molar excess BMH over apoE(201–299), respectively.