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. 2021 Jun 17;12:3709. doi: 10.1038/s41467-021-24110-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Illustration of workflow of scRNA-seq in human normal scar and fibrotic skin disease dermis samples. b Unbiased clustering of 40655 cells reveals 21 cellular clusters. Clusters are distinguished by different colors. The general identity of each cell cluster is shown on the right. c Unsupervised hierarchical clustering of average gene expression showing relatedness of cell clusters (correlation distance metric, average linkage). d Heatmap of differentially expressed genes. For each cluster, the top 10 genes and their relative expression levels in all sequenced cells are shown. Selected genes for each cluster are color-coded and shown on the right. e Feature plots of expression distribution for selected cluster-specific genes. Expression levels for each cell are color-coded and overlaid onto the UMAP plot. f The proportion of cell lineages in keloids (KL) and normal scars (NS). g Number of differentially expressed (DE) genes in each cell type with >100 cells available in keloid and normal scars (two-sided Wilcoxon Rank Sum test, Bonferroni correction, log fold change (FC) cutoff of 0.5, and adjusted P-value of <0.05). Red bars indicate upregulated genes, and blue bars indicate downregulated genes in keloid.