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. 2021 Jun 17;12:3709. doi: 10.1038/s41467-021-24110-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Isolation of CD266⁺CD9⁻ and other fibroblasts from keloid dermis by flow cytometry. b qRT-PCR assay of some mesenchymal fibroblast marker genes expression in CD266⁺/CD9⁻ fibroblasts and other fibroblasts from keloid dermis. Data are presented as mean values ± SD (n = 3 biologically independent experiments.). Two-sided unpaired t-test, ***P < 0.001(ASPN:P = 0.00003, COL11A1:P = 0.00019, POSTN:P = 0.00004, ADAM12:P = 0.00015, P311:P = 0.00019, COMP:P = 0.00002). c Western blot assay of mesenchymal fibroblast marker genes expression in CD266⁺/CD9⁻ fibroblasts and other fibroblasts from keloid dermis. The experiments were repeated three times with three different fibroblast donors, and here a representative result was shown. d GO Biological Process enrichment analysis of differentially expressed genes between keloid CD266⁺/CD9⁻ fibroblasts and other fibroblasts. e GSEA enrichment plots for representative signaling pathways upregulated in keloid CD266⁺/CD9⁻ fibroblasts compared to other fibroblasts. (NES normalized enrichment score, corrected for multiple comparisons using FDR method, P-value were showed in plots). f, g Keloid other fibroblasts were treated with the supernatant of CD266⁺/CD9⁻ or other fibroblasts. The expression of collagen I and collagen III was analyzed by qRT-PCR (f) or western blot (g). Data are presented as mean values ± SD (n = 3 biologically independent experiments). Two-sided unpaired t-test, **P < 0.01, ***P < 0.001. (COL1A1: P = 0.000009 and P = 0.000015, respectively; COL3A1: P = 0.00006 and P = 0.00214, respectively). The experiments were repeated three times with three different fibroblast donors, and here a representative result was shown. h Keloid other fibroblasts were treated as indicated in the figure. The expression of collagen I and collagen III was analyzed by western blot. The POSTN antibody neutralizing experiments were repeated three times with three different fibroblast donors, and here a representative result was shown.