Fig. 5. EEP-GFP-HiBiT fusion proteins exhibit punctate staining, suggesting limited endosomal escape.

HEK293-LSA cells treated with 1 µM EEP-GFP-HiBiT (green) proteins: a GFP, b R9, c TAT, d ZF5.3, e E5TAT, f 5.3, g pHD118, h pHlip and i HA2 at 1 µM for 4 h. Cells were washed and nuclei were stained with Hoechst 33342 (pseudocoloured blue) before confocal microscopy. n = 2 independent experiments Scale bar = 10 µm.