Skip to main content
. 2021 Jun 17;12:3689. doi: 10.1038/s41467-021-23884-5

Fig. 4. Multiscale, multi-modal imaging.

Fig. 4

a Imaging chamber with stabilizer in place under ambient illumination, approximately two weeks after implant. b Cortical surface imaged using widefield (1P) imaging (representative example expression photo taken from the start of each imaging session). c Zoomed in region highlighted in green box in b. Vascular landmark used to calibrate microscope stage positions indicated with blue arrow. d Further zoomed widefield image showing microvascular features used for localizing 2P FOVs and aligning 2P imaging with CLARITY, marked with magenta arrows. e 2P image acquired from the same FOV as d. vascular landmarks marked with purple arrows. f CLARITY volume from the same FOV in d and e (anti-GCaMP antibody labeling green, vasculature white, results from a single ex vivo imaging session). g, h Two example fields of view including neural processes and L2/3 cell bodies ~250 µm below the surface vasculature (monkey W, example images from one of ~20 imaging sessions).