Figure 2.

Granulosa cell characteristics after collagenase dissociation. (A) Bright field images of granulosa cells adhering to plate surface following enzymatic dissociation and overnight culture. Images taken at 40× magnification, scale bar equivalent to 50 μm, (B) granulosa cell yield by number of ovaries dissociated in a single replicate with linear fit, and (C) RNA yield (ng per 100 000 cells) by age of ovaries dissociated. Data presented is from 25 dissociation replicates, 11 utilising PND1 ovaries, and 14 replicates of PND4 ovaries. NSD, no significant difference; PND, days post birth.