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. 2021 May 10;27(6):gaab033. doi: 10.1093/molehr/gaab033

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© The Author(s) 2021. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Gene expression of dissociated cells using the papain dissociation method. (A) Expression of selected ovarian cell-specific markers determined via RNA sequencing of papain-dissociated cells originating from embryonic day 18.5, postnatal Day 8 and postnatal Day 12 week-old ovaries (n = 4 samples per group). Granulosa cell markers (Foxl2 and Gata4) in purple, Oocyte markers (Ddx4 and Gdf9) in green, and theca cell marker (Gli3) in blue. (B) Expression of selected theca cell-specific markers (Ptch1, Gli2, Cyp19a1, and Cyp17a1) determined via RNA sequencing of papain-dissociated cells originating from both embryonic Day 18.5, postnatal Day 8 and postnatal Day 12 week-old ovaries (n = 4 samples per group). TPM stands for transcripts per million mapped reads and refers to the gene expression value generated via RNA sequencing.